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Assay Development and High-Throughput Screening

The goal of the NCGC Assay Biology Teams in the NCATS Division of Pre-Clinical Innovation is to optimize biochemical, cellular and model organism-based assays requested or submitted by the biomedical research community for high-throughput small molecule screening. The results of these screens (probes) can be used to further interrogate protein and cell functions and biological processes relevant to physiology and disease. In addition, these probes can be further optimized to become potential lead candidates in the drug development pipeline.

The NCGC Assay Guidance Manual and the High-Throughput Screening Assay Guidance Criteria contain detailed information on developing appropriate assays for high-throughput screening projects.

96-well, 384-well and 1,536-well plates. With improved microfluidics technology, plates with more wells can be used, allowing screening of more samples per pass.

NCATS at Work

With support from the Michael J. Fox Foundation for Parkinson's Research, experts from NCATS and the National Institute of Neurological Disorders and Stroke are leading a project using NCATS' chemical screening resources to advance development of potential therapeutics for a broad range of diseases.

Assay Guidance Manual

Cover of Assay Guidance Manual

NIH and Eli Lilly publish guide to help researchers develop therapeutic screening tests